Journal: PLoS ONE
Article Title: HP0197 Contributes to CPS Synthesis and the Virulence of Streptococcus suis via CcpA
doi: 10.1371/journal.pone.0050987
Figure Lengend Snippet: ( A ) Microarray-based comparative transcriptomic analysis of Δ hp0197 and WT strains. A hierarchical cluster of 193 transcripts in the Δ hp0197 compared to the WT strain. The increased and decreased transcript expression levels were indicated by red and green, respectively. ( B ) Different expression profiles identified by microarray analysis were validated by qRT-PCR based on the data obtained from a total of 12 genes. From left to right: ssu05_1372, ssu05_1933, ssu05_0167, ssu05_2076, ssu05_2137, ssu05_1039, ssu05_0360, ssu05_1401, ssu05_0812, ssu05_0573 ( cps 2J ), ssu05_0265 and ssu05_0469. ( C ) Functional classification of the differentially expressed genes. J: Translation, ribosomal structure and biogenesis; K: Transcription; L: Replication, recombination and repair; D: Cell cycle control and cell division; V: Defense mechanisms; T: Signal transduction mechanisms; M: Cell membrane and envelope biogenesis; U: Intracellular trafficking, secretion and vesicular transport; O: Posttranslational modification, protein turnover, chaperones; C: Energy production and conversion; G: Carbohydrate transport and metabolism; E: Amino acid transport and metabolism; F: Nucleotide transport and metabolism; H: Coenzyme transport and metabolism; I: Lipid transport and metabolism; P: Inorganic ion transport and metabolism; Q: Secondary metabolites biosynthesis, transport and catabolism; R: General function prediction only; S: Function unknown; Not: Not in COGs.
Article Snippet: Based on the whole genome sequence of 05ZYH33, specific 40- to 60-mer oligo-nucleotides were designed to cover every putative ORF (2194 in total) in the genome using the eArray EArray Online Microarray Design Tool ( https://earray.chem.agilent.com/earray/ ) and were printed six times on the surface of each microarray slide (Agilent).
Techniques: Microarray, Expressing, Quantitative RT-PCR, Functional Assay, Transduction, Modification